human gb cell lines a172  (ATCC)

Journal: bioRxiv

Article Title: Intraventricular infusion to circumvent the blood-brain barrier to gemcitabine

doi: 10.64898/2026.05.01.722145

Figure Lengend Snippet: GB cells were exposed to GEM for 1h or 24 h at the indicated concentrations. After GEM exposure, cells were maintained in drug-free fresh culture medium for 72 h before a viability assay with Crystal Violet. IC50 of GEM on the A172, U87-MG and U118-MG human GB cell lines were indicated in the legend of . Phase contrast microscopy images of GB cells treated for 24 h at IC 90. Cells were maintained in culture in drug free medium and observed 24 h or 5 days after GEM treatment. Note the presence of enlarged / flattened cells.

Article Snippet: Human GB cell lines A172, U87-MG and U118-MG were purchased from American Type Culture Collection (ATCC) (LGC Standards, Strasbourg, France) and cultured in Dulbecco Modified Eagle Medium (DMEM) supplemented with 10% fetal calf serum (Jacques Boy, Reims, France), 2 mM glutamine and penicillin / streptomycin.

Techniques: Viability Assay, Microscopy

Journal: Oncology Research

Article Title: Assessing the impact of CD73 inhibition on overcoming anti-EGFR resistance in glioma cells

doi: 10.32604/or.2024.055508

Figure Lengend Snippet: EGFR expression in human glioblastoma lines. (A) median fluorescence intensity (MFI) (B) percentage of cells expressing EGFR. Expression analysis was performed by flow cytometry. (C) Viable U251-MG cells exposed to treatment with Tyrphostin AG1478 for 48 h. (D) Cell morphology of U251-MG cells in the control group (Scale bar = 100 µm) (left) and in the AG1478-treated cells (right). Image obtained by microscopy. (E) Hemolysis of red blood cells treated with Tyrphostin AG1478 for 48 h. (F) U251-MG cells treated with Tyrphostin AG1478 and Temozolomide for 48 h. Cell viability analyses were performed by counting with Trypan Blue. * p < 0.05; ** p < 0.01: *** p < 0.001; **** p < 0.0001.

Article Snippet: The U251-MG, U87-MG, U138-MG, T98G, and A172 human GB cell lines were acquired from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Expressing, Fluorescence, Flow Cytometry, Control, Microscopy

Journal: Oncology Letters

Article Title: Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells

doi: 10.3892/ol.2017.7518

Figure Lengend Snippet: Effect of SB on the proliferation of A172 human glioblastoma cells. (A) In vitro proliferation assay of A172 cells following treatment with different concentrations of SB (0.0, 0.5, 1.0, 2.0 and 4.0 mM). Results are presented as the mean ± standard deviation (n=3). *P<0.01 vs. treatment with 0 mM SB. (B) Reversibility of SB inhibitory effect. A total of 4 days following treatment with 2 mM SB, A172 cells were washed with media without SB and cultured for 4 additional days. The results are presented as the mean ± standard deviation (n=3). *P<0.01 vs. treatment with 2 mM SB. (C) Cell cycle analysis of the A172 cells in (B) using a BD FACSCalibur system. SB, sodium butyrate.

Article Snippet: The human GB cell line A172 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: In Vitro, Proliferation Assay, Standard Deviation, Cell Culture, Cell Cycle Assay

Journal: Oncology Letters

Article Title: Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells

doi: 10.3892/ol.2017.7518

Figure Lengend Snippet: Effect of SB and the HDAC inhibitor TSA on SA β-gal staining. (A) A172 cells treated with SB (0–4 mM) or TSA (25–100 nM) for 4 days were stained with SA β-gal. β-gal-positive cells are indicated by white arrows (scale bar=20 µm). (B) β-gal-positive cells in (A) were analyzed and counted. Results are presented as the mean ± standard deviation (n=4); *P<0.01 vs. control. SA β-gal, senescence-associated β-galactosidase; SB, sodium butyrate; HDAC, histone deacetylase; TSA, trichostatin A.

Article Snippet: The human GB cell line A172 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Staining, Standard Deviation, Control, Histone Deacetylase Assay

Journal: Oncology Letters

Article Title: Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells

doi: 10.3892/ol.2017.7518

Figure Lengend Snippet: Western blot and RT-PCR analyses of p21 (FK506 binding protein like), p27 (cyclin dependent kinase inhibitor 1B) and p53 mRNA and protein expression in 2-mM SB-treated A172 cells. (A) A172 cells were treated with 2 mM SB for the indicated time and the protein levels of the cell cycle regulators p21, p27 and p53 were analyzed by western blot analysis. (B) A172 cells were treated with 2 mM SB for the indicated time and mRNA levels of p21, p27 and p53 were analyzed by RT-PCR. RT-PCR, reverse transcription-polymerase chain reaction; SB, sodium butyrate; bp, base pairs.

Article Snippet: The human GB cell line A172 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Binding Assay, Expressing, Reverse Transcription, Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells

doi: 10.3892/ol.2017.7518

Figure Lengend Snippet: Effect of SB on the motility, invasion and morphology of A172 cells. (A) Upper panel: A172 cells were treated with the indicated concentrations (0–4 mM) of SB for 48 h and then cell motility was analyzed using with 0.5% FBS as a chemoattractant. Results are presented as the mean ± standard deviation (n=6). Lower panel: Cell invasion was analyzed with 0.5% FBS as a chemoattractant using Matrigel-coated chambers. Results are presented as the mean ± standard deviation (n=3). *P<0.01 vs. control. (B) A172 cells were treated with the indicated concentrations (0–4 mM) of SB for 48 h and then the phosphorylation of FAK and MLC20 were analyzed by western blotting. (C) A172 cells were treated with the indicated concentrations (0–4 mM) of SB for 48 h and then cell spreading was analyzed by measuring the cell area. Results are presented as the mean ± standard deviation (n=100). *P<0.01 vs. control. SB, sodium butyrate; FBS, fetal bovine serum; FAK, focal adhesion kinase; MLC, myosin light chain; p-, phosphorylated.

Article Snippet: The human GB cell line A172 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Standard Deviation, Control, Phospho-proteomics, Western Blot

Journal: Oncology Letters

Article Title: Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells

doi: 10.3892/ol.2017.7518

Figure Lengend Snippet: Effect of SB on A172 cell morphology and intracellular FAK and MLC20 phosphorylation. Immunostaining of A172 cells treated with the indicated concentrations (0–4 mM) of SB for 48 h using phospho-specific pY397 FAK antibody. White arrows indicate the focal adhesions at the cell peripheral regions. Scale bar=20 µm. SB, sodium butyrate; FAK, focal adhesion kinase; MLC, myosin light chain.

Article Snippet: The human GB cell line A172 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Phospho-proteomics, Immunostaining

Journal: Oncology Letters

Article Title: Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells

doi: 10.3892/ol.2017.7518

Figure Lengend Snippet: Reversibility of the effects of SB in A172 cells. (A) At 4 days after treatment with 2 mM SB, A172 cells were washed in media without SB and cultured for 4 additional days. The effects on the expression of p21 (FK506 binding protein like), p27 (cyclin dependent kinase inhibitor 1B), p53, SIRT1, pY397/pY577 FAK and p-MLC20 were determined by western blotting. (B) Cell motility was measured in A172 cells treated as in (A). Results are presented as the mean ± standard deviation (n=6). *P<0.01 vs. control. SB, sodium butyrate; SIRT, sirtuin; FAK, focal adhesion kinase; p-, phosphorylated; MLC, myosin light chain.

Article Snippet: The human GB cell line A172 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Cell Culture, Expressing, Binding Assay, Western Blot, Standard Deviation, Control

Journal: Oncology Letters

Article Title: Sodium butyrate induces senescence and inhibits the invasiveness of glioblastoma cells

doi: 10.3892/ol.2017.7518

Figure Lengend Snippet: Effect of SB on the levels of p21 (FK506 binding protein like), p53, p27 (cyclin dependent kinase inhibitor 1B), p16 and pFAK in p21 knockdown and control A172 cells. (A) Knockdown of p21 was achieved by transfection with specific siRNA against human p21 for 72 h. The effect of 2 mM SB on the levels of p21 (FK506 binding protein like), p53, p27 (cyclin dependent kinase inhibitor 1B), p16 and FAK phosphorylation in control and p21-knockdown cells was determined using western blot analysis. (B) β-galactosidase positive staining (n=4; upper panel); and cell motility (lower panel; n=6) were measured in the A172 cells in (A). Results are presented as the mean ± standard deviation. *P<0.01 vs. control. (C) Cell cycle analysis of the A172 cells in (A) using a BD FACScalibur system. SB, sodium butyrate; p-, phosphorylated; FAK, focal adhesion kinase; si, small interfering; C, control.

Article Snippet: The human GB cell line A172 was obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Binding Assay, Knockdown, Control, Transfection, Phospho-proteomics, Western Blot, Staining, Standard Deviation, Cell Cycle Assay